January 19- 20

Jan uary 19

Checking the result of the whole antibiotic sensitivity experiment. Bacteria 33 has been cultured.

The process of culturing bacteria 33:
materials: two autoclaved 500ml flasks, bacteria sample, schott bottle, nutrient broth powder, sea water, inoculating loop, parafilm, ethanol, tissues, aluminium foil and permanent marker.

The original petri dish that used to grow bacteria 33 has been autoclaved/ destroyed, thus we need to get the bacteria sample from the broth. However, the chances for the bacteria to be alive in the broth is very little thus we can only hope for the best.

1. Prepare 500ml marine nutrient broth in the Schott Bottle. Autoclave it for 1 hour and 30 minutes to ensure no other living little organisms in the broth and the bottle. Once all is done, we are ready to move the broth into two autoclaved flasks.

2. Clean the laminar flow by using the usual method- spraying the ethanol on the surface and wipe it with tissues or cloth. Arrange everything nicely and put things that are needed into the clean laminar flow.

3. Open the cover of the Schott bottle and flame the mouth with fire. At the same time, open the aluminium foil of the autoclaved flask and flame the mouth with fire too. Pour 250 ml marine nutrient broth into the flask.

4. Close the mouth of the flask immediately with aluminium foil and let it cool down to room temperature. Do the same for the second flask.

5. Flame the inoculating loop with fire. Open the flask that contains

3. Because of the high contamination that has affected the results, thus decision has been made to restart doing this whole thing again by beginning from the very first step- sampling.

As Chinese New Year holidays approaching so the project will be continued after the Chinese New Year break, which is after February 5.