January 12

Title of Experiment: Culturing Bacteria into Nutrient Broth

Objectives: To testify the existence of samples of mucus given and to transfer the bacteria sample from petri dishes into nutrient broth medium.

Materials: nutrient broth medium in flasks, Two sample of culture microbe (labeled as 11 and 33), inoculating loop, parafilm, aluminium foils, fire flame, ethanol, tissues and permanent marker.

*Note that the culture microbes of bacteria were chosen according to its growing rate.

Method:
1. Clean the laminar flow by spraying ethanol on its surfaces and wipe it with the tissues. Switch on the blower and light the fire flame after that.
2. Move the materials into laminar flow and put it closer/around the flame. Label the flask (sterilized) 11 and 33 with researcher's name and the date.
3. Sterilize the inoculating loop by flaming it in the fire. Leave it to cool down. At the same time. remove the parafilm from the petri dish.
4. Open the petri dish gently and place the tip of the inoculating loop gently on the surface that has growing sign (it looks like white lines with gelly like on it) . Streak the tip on it slightly.
5. Close the petri dish and opent he sterilized flask immediately and put the tip of the inoculating loop into the nutrient broth immediately. Stir it if possible. Close it back with new aluminium foil.
6. Flame the loop immediately.
7. Repeat step 3-6 for the other culture microbe.
8. Put the flasks to shaker and shake around 13,000 rpm - 15,000 rppm for 24 hours in ambient temperature.

Result: The nutrient broth turn into blown blurrish color-- growing signs of bacteria
















































































Conclusion: The growth bacteria shows that the bacteria is active. The broth is ready to be test for its antibiotic sensitivity.