March 11

Experiment: Isolating Bacteria from Fish Mucus

Objective: To grow bacteria from the fish mucus to get pure culture

Materials:

The four Petri dishes contains fish mucus from last experiment, 20 empty petri dishes with MNA, inoculating loop, fireflame, and parafilm.

Method:

1. Clean the laminar flow using method from the previous experiment (UV first and then ethanol
method)
2. Put all the materials in the laminar floww.
3. Use permanent marker make a mark at the surface of the petri dish to the colony that has
been picked for isolating.
4. Give identical names for each of the colony been picked. For example, the first colony picked
from K1A shall called K1AI and then K1A2 and etc. Label the new petri dish contains MNA
too. Isolating method can only be started after labeling and marking all the colonies and Petri
dishes accordingly.
5. Flame the inoculating loop and let it cool down. Lets start with K1A. Open the Petri dish of
K1A and use the loop to take a drop of from the colony selected. Be careful not to touch the
loop with other things as contamination usually start from there.
6. Streak the bacteria from the colony to the labeled Petri dish earlier. Follow the four method of
streaking. Flame the loop for each step of streaking.
7. Repeat step 5 and 6 for other colonies.
There were 17 plates of bacteria isolated from the four Petri dishes that contains all bacteria from the fish mucus, five from KlA, and four from K1B, S1A and S1B.

The culture is then left in room temperature for 24 hours for it to grow.

Result:

Five from KlA
























































































Four from K1B







































































Four from S1A





































































And four from S1B






































































There were various kind of bacteria can be seen growing in the Petri dish with different colors and different shapes. These are unknown bacteria and now the next step is to identify them. However, before we can proceed to find out what kind of species these bacteria are we need to test for its antibacterial sensitivity. The existence of antibacterial sensitivity can help the researcher in the process of characterization and identification.

Conclusion: There were more than one kind of bacteria in a sample of fish mucus and each of them are identified as different when they grow in different colonies and different pigment. The next step is to do antibacterial sensitivity.